Characterization of Two Novel FMRP Interacting Proteins
Jean-Louis Mandel, PhD
Principal Investigator
Barbara Bardoni, PhD
FRAXA Fellow
University of Strasbourg
Strasbourg, France
2000 Grant Funding: $30,000
Summary
Dr. Jean-Louis Mandel and his team at the University of Strasbourg studied the function of two proteins to better understand the affects of the absence of the FMR1 expression.
The Science
Search for FMRP (Fragile X Protein) Cellular Function Through the Characterization of Two Novel FMRP Interacting Proteins
by Barbara Bardoni, 1/1/2000
We have searched for new proteins that interact with the Fragile X protein FMRP using a technique called the two-hybrid assay in yeast. After screening a mouse embryonic (E9.5-EE12.5) library, we found two novel proteins that we are currently characterizing: NUFIP1 (Nuclear FMRP Interacting Protein) and CYFIP (Cytoplasmic FMRP Interacting Protein). Understanding the functions of these new proteins is an essential step in the definition of the molecular and developmental mechanisms by which the absence of FMR1 expression causes Fragile X syndrome.
